Contact an Account ManagerSeparation and quantitative determination of high-molecular-weight subunits of glutenin from different wheat varieties
Summary
Type: HPLC
Application #: 108450
Detection: UV, 220nm
Conditions: temperature 60°C
left: elution system 1, eluent A, B, C 29, 34 and 80% acetonitrile and 0.1% TFA, respectively, linear gradient 0 - 20min 0 - 20% B, 20 - 180min 20 - 100% B, 100% C for rinsing
right: elution system 2a, eluent A: 15% 2-propanol, 0.1% TFA, eluent B: 80% acetonitrile, 0.1% TFA, linear gradient 0 - 10min 6 - 19% B, 10 - 85min 19 - 37.5% B, 85 - 105 min 37.5 - 62.5% B
flow rate 1ml/min
Authors: Seilmeier W
Source: Z Lebensm Unters Forsch 192 (1991) 124 - 129
Details
Legend: Fig. 1 and 2. Chromatograms of HMW subunits of glutenin from the wheat variety
Farmer.